Chromatographic analysis in bacteriological diagnostics of blood cultures, exudates and bronchoalveolar lavages

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Welcome

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Chromatographic analysis in bacteriological diagnostics of blood cultures, exudates and bronchoalveolar lavages Jaroslav Julák, Vlasta Rosová, Eva StránskáCharles University, Prague

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Volatile acids (VFA) produced by bacterial fermentation

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Mechanisms of SPME extraction Start Equilibrium Absorption Adsorption in pores

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SPME extraction procedure

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SPME fibres

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SPME of VSFA-2 standard mixture

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SPME of exudates containing anaerobic and aerobic bacteria

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VFA patterns of reference strains on different media (as peak height / I.S. ratio)

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GC-MS of TBS-VFA (sample and standard)

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Identification of cyclohexanone (GC-MS of TBS derivatives)

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Isomers of methylbutyric acid (GC-MS of TBS derivatives)

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Identification of cyclohexanone (GC-MS of native samples)

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SIFT (Selected Ion Flow Tube Mass Spectrometry) apparatus

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Neutral and acidic compounds detected in selected BAL samples by SIFT

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Detection of bacteria using chromatography of VFA – References• Julák J.: Identification of bacteria by instrumental chemical analysis (in Czech). Karolinum, Praha, 1998• Julák J., Rosová V., Procházková-Francisci E., Stránská E.: Evaluation of blood cultures by volatile fatty acids determination (in Czech). Klin. mikrobiol. inf. lék. 5, 262-263 (1999)• Julák J., Stránská E., Procházková-Francisci E., Rosová V.: Blood cultures evaluation by gas chromatography of volatile fatty acids. Med. Sci. Monit. 6, 605-610 (2000)• Julák J., Stránská E., Rosová V., Procházková-Francisci E.: Solid phase microextraction for microbiological diagnostics of exudates (in Czech). Klin. mikrobiol. inf. lék. 8, 135-137 (2002) • Julák J., Procházková-Francisci E., Stránská E., Rosová V.: Evaluation of exudates by solid phase microextraction-gas chromatography. J. Microbiol. Meth. 52, 101-108 (2003)• Julák J.: Sepsis and purulent diseases diagnostics by chemical analysis (in Czech). Bull. cs. spol. mikrobiol. 34, 93-97 (2003)• Julák J., Stránská E., Rosová V., Dohnalová A.: Chemical analysis of bronchoalveolar lavage in diagnostics of pneumonia (in Czech). Klin. mikrobiol. inf. lék. 10, 279-283 (2004)• Julák J.: Chromatographic analysis in bacteriologic diagnostics of blood cultures, exudates, and bronchoalveolar lavages. Prague Med. Rep. 106, 175-194 (2005)• Julák J., Stránská E., Rosová V., Geppert H., Španel P., Smith D.: Bronchoalveolar lavage examined by solid phase microextraction, gas chromatography-mass spectrometry and selected ion flow tube mass spectrometry. J. Microbiol. Meth., 65, 76-86, (2006)

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PowerPoint Slide Presentation on Summarizes previously published results achieved in bacteriologica more PowerPoint Slide Presentation on Summarizes previously published results achieved in bacteriological diagnostics of blood cultures, suppurative and apyogenous exudates and bronchoalveolar lavages (BAL) using solid phase microextraction (SPME)gas chromatography (GC)mass spectrometry (MS). Up to 20 % clinical cases appeared to be false negative by cultivation with respect to anaerobes. less
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