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| PCR alternates between two phases: separate DNA into single strands using heat; PCR rapidly amplifies a single DNA molecule into billions of molecules |
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| Real Time PCR and Application in Medicine |
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| Amplification of genomic DNA with the Polymerase Chain Reaction (PCR) Amplification of complementary DNA (cDNA) with the Polymerase Chain Reaction |
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| Real-time PCR monitors the fluorescence emitted during the reaction as an indicator of amplicon production at each PCR cycle (in real time) as opposed to |
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| Quantitative Real-Time PCR is an important technique for quantifying messenger RNA levels (gene expression) and DNA gene levels (copy number) in biological |
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| Gene expression analysis of macrophages housing cell-cycling Leishmania amastigotes using microarray hybridization and real-time PCR assays |
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| Equipment for organics: GCMS, other forms of chromatography/mass spectrometry . Equipment for microbiology: anaerobic culturing lab, PCR, nucleic acid extraction, microscopy, standard |
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| neither special DNA manipulations before PCR (restriction digestion, ligation, etc) nor laborious screening afterward (Southern hybridization, |
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| “Beginning with a single molecule of the genetic material DNA, the PCR can generate 100 billion similar molecules in an afternoon. |
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| The polymerase chain reaction (PCR) can selectively and rapidly amplify a given DNA 3) PCR is used in forensic medicine to generate a DNA fingerprint |
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